KOOBAN IYO SHARAXA IMTIXAANKA
Chikungunya waa caabuq naadirka ah oo fayras ah oo ay gudbiso qaniinyada kaneecada Aedes aegypti ee cudurka qabta.Waxaa lagu gartaa finan, qandho, iyo xanuunka kalagoysyada oo aad u daran (arthralgias) kaas oo inta badan socda saddex ilaa todoba maalmood.Magaca waxaa laga soo qaatay ereyga Makonde ee macnihiisu yahay "ka soo laabta" iyada oo la tixraacayo qaabka foorarsan ee loo sameeyay natiijada calaamadaha arthritic ee cudurka.Waxay ka dhacdaa xilli-roobaadka meelaha kulaylaha ah ee adduunka, oo ay ugu horreeyaan Afrika, Koonfur-bari Aasiya, Koonfurta Hindiya iyo Pakistan.Calaamaduhu inta badan waa qaab kiliinikada aan la kala saari karin kuwa lagu arkay qandhada dengue.Runtii, infekshannada laba-geesoodka ah ee dengue iyo chikungunya ayaa laga soo sheegay Hindiya.Si ka duwan dengue, calaamadaha dhiigbaxa ayaa ah kuwo naadir ah inta badana cudurku waa jirro xumad iskeed u xaddidaysa.Sidaa darteed aad bay muhiim u tahay in kiliinikada laga sooco dengue iyo caabuqa CHIK.CHIK waxaa lagu ogaadaa iyadoo lagu salaynayo falanqaynta serological iyo fayraska go'doominta ee jiirarka ama dhaqanka unugyada.IgM immunoassay waa habka tijaabada shaybaadhka ugu waxtarka badan.Tijaabada degdega ah ee Chikungunya IgG/IgM waxay isticmaashaa antigens-ka dib-u-habaynta ah ee laga helay borotiinka qaab-dhismeedkeeda, waxay ogaataa IgG/IgM anti-CHIK ee serum ama balaasmaha bukaanka 20 daqiiqo gudahood.Baaritaanka waxaa sameyn kara kuwa aan tababarin ama
shaqaale xirfad yar leh, oo aan haysan qalab shaybaar oo dhib badan.
MABADA
Tijaabada degdega ah ee Chikungunya IgG/IgM waa immunoassay chromatographic socodka dambe.Cajalka imtixaanku waxa uu ka kooban yahay: 1) suuf isku xidhan oo midab leh oo burgundy ah oo ay ku jiraan Chikungunya recombinant envelope antigens oo ay ku xidhan yihiin dahab kolloid ah (dengue conjugates) iyo bakaylaha IgG-dahab conjugates,2) xabxab nitrocellulose ah oo ka kooban laba qaybood oo tijaabo ah (G iyo M bands) iyo band control).Kooxda G waxa hore loogu dahaadhay antibody si loo ogaado IgG anti-Chikungunya virus, M band waxa lagu dahaadhay antibody si loo ogaado IgM anti-Chikungunya fayraska, iyo kooxda C waxa hore u dahaarka leh ariga ka hortagga bakaylaha IgG.
Marka mug ku filan muunada shaybaadhka lagu shubo ceelka muunada ee cajalada tijaabada, muunada waxa ay u guurtaa ficil capillary ee guud ahaan cajalada.Fayraska ka hortagga Chikungunya ee IgG haddii uu ku jiro muunada waxa uu xidhi doonaa isku-xidhayaasha Chikungunya.Reagents lambarrada dufcadaha kala duwan looma isticmaali karo si isku beddelasho ah. Immunocomplex-ka ayaa markaa qabta reagenti ku dahaaran G band, oo samaynaya koox midab leh oo Burgundy ah, taas oo muujinaysa fayraska Chikungunya ee IgG natiijada baadhista togan oo soo jeedinaysa infekshan dhawaan ama soo noqnoqda.Fayraska ka hortagga Chikungunya ee IgM, haddii uu ku jiro muunada, waxa uu xidhi doonaa isku-xidhayaasha Chikungunya.Immunocomplex-ka ayaa markaa qabta reagent-ka hore ugu dahaadhay kooxda M, oo samaynaysa koox M-midab leh oo burgundy ah, taasoo muujinaysa fayraska Chikungunya ee IgM natiijada baadhista togan oo soo jeedinaysa infekshan cusub.Maqnaanshaha koox kasta oo tijaabo ah (G iyo M) waxay soo jeedinaysaa natiijo taban. Tijaabadu waxay ka kooban tahay koontarool gudaha ah (C band) kaas oo ah inuu soo bandhigo koox midab leh oo burgundy ah oo ah difaaca difaaca ee riyaha ka hortagga bakaylaha IgG/bakaylaha IgG-dahabka iyadoo aan loo eegin horumarka midabka mid ka mid ah kooxaha T.Haddii kale, natiijadu waa sax, muunadana waa in lagu tijaabiyaa qalab kale.
