Chikungunya IgG/IgM+NS1 Qalabka Tijaabada Degdegga ah ee Antigen-ka (Dahab Colloidal)

Chikungunya waa caabuq naadirka ah oo fayras ah oo ay gudbiso qaniinyada kaneecada Aedes aegypti ee cudurka qabta.Waxaa lagu gartaa finan, qandho, iyo xanuunka kalagoysyada oo aad u daran (arthralgias) kaas oo inta badan socda saddex ilaa todoba maalmood.Magaca waxaa laga soo qaatay ereyga Makonde ee macnihiisu yahay "ka soo laabta" marka loo eego qaabka foorarsan ee loo sameeyay natiijada calaamadaha arthritic ee cudurka.Waxay dhacdaa xilli roobaadka

meelaha kulaylaha ah ee dunida, ugu horrayn Afrika, Koonfur-Bari Aasiya, koonfurta Hindiya iyo Pakistan.Calaamaduhu inta badan waa qaab kiliinikada aan la kala saari karin kuwa lagu arkay qandhada dengue.Runtii, infekshannada laba-geesoodka ah ee dengue iyo chikungunya ayaa laga soo sheegay Hindiya.Si ka duwan dengue, calaamadaha dhiigbaxa ayaa ah kuwo naadir ah inta badana cudurku waa jirro xumad iskeed u xaddidaysa.Sidaa darteed aad bay muhiim u tahay

si loo kala saaro dengue iyo caabuqa CHIK.CHIK waxaa lagu ogaadaa iyadoo lagu salaynayo falanqaynta serological iyo fayraska go'doominta ee jiirarka ama dhaqanka unugyada.IgM immunoassay waa habka tijaabada shaybaadhka ugu waxtarka badan.Tijaabada degdega ah ee Chikungunya IgG/IgM waxay isticmaashaa antigens-ka dib-u-soo-celinta ee laga helay borotiinka qaab-dhismeedkeeda, waxay ogaataa IgG/IgM anti-CHIK ee serum ama balaasmaha bukaanka 20 daqiiqo gudahood.Baaritaanka waxaa sameyn kara kuwa aan tababarin ama

shaqaale xirfad yar leh, oo aan haysan qalab shaybaar oo dhib badan.

Tijaabada degdegga ah ee Chikungunya IgG/IgM+NS1 antigen-ka waa unug-socod-dambeed oo chromatographic immunoassay ah.Cajalka imtixaanku wuxuu ka kooban yahay IgG/IgM Strip iyo NS1 strip.

IgG/IgM strip: 1) suuf conjugate ah midabka burgundy oo ay ku jiraan Chikungunya recombinant enveloped antigens conjugated dahab kolloid ah (dengue conjugates) iyo bakayle IgG-dahab conjugates,2) xuubka nitrocellulose oo ka kooban laba qaybood oo tijaabo ah (G iyo M bands) iyo a band control (C band).Kooxda G waxa hore loogu dahaadhay antibody si loo ogaado IgG anti-Chikungunya virus, M band waxa lagu dahaadhay antibody si loo ogaado IgM anti-Chikungunya fayraska, iyo kooxda C waxa hore u dahaarka leh ariga ka hortagga bakaylaha IgG.

NS1 strip:1) suuf conjugate ah midabka burgundy oo ay ku jiraan mouse anti-Chikungunya NS1 antigen lagu daray dahab kolloid ah (Chikungunya Ab conjugates), 2) xariijimo nitrocellulose ah oo ka kooban band tijaabo ah (T band) iyo band kontorool (C band).Kooxda T waxaa horay loogu dahaadhay anti-Chikungunya NS1 antigen bakayle, iyo kooxda C waxaa horay loogu dahaadhay anti-jiirka IgG antibody.

Xariijimaha IgG/IgM: Marka mug ku filan muunada shaybaadhka lagu shubo muunada ceelka cajalada tijaabada, muunadu waxa ay u guurtaa ficil capillary ee cajaladda oo dhan.Fayraska ka hortagga Chikungunya ee IgG haddii uu ku jiro muunada waxa uu xidhi doonaa isku-xidhayaasha Chikungunya.Immunocomplex-ka ayaa markaa qabta reagenti ku dahaadhay kooxda G, oo samaynaysa koox G midab leh oo burgundy ah, taasoo muujinaysa fayraska Chikungunya ee IgG natiijada baadhista togan oo soo jeedinaysa caabuq dhawaan ama soo noqnoqda.Fayraska ka hortagga Chikungunya ee IgM, haddii uu ku jiro muunada, waxa uu xidhi doonaa isku-xidhayaasha Chikungunya.Immunocomplex-ka ayaa markaa qabta reagent-ka hore ugu dahaadhay qaybta M, oo samaynaysa koox M-midab leh oo burgundy ah, taasoo muujinaysa fayraska Chikungunya ee IgM natiijada baadhista togan oo soo jeedinaysa infekshan cusub.Maqnaanshaha koox kasta oo tijaabo ah (G iyo M) waxay soo jeedinaysaa natiijo taban. Tijaabadu waxay ka kooban tahay kontoroolka gudaha (C band) kaas oo soo bandhigaya koox midab leh oo burgundy ah oo ah difaaca difaaca ee riyaha ka hortagga bakaylaha IgG/

bakayle IgG-dahab conjugate iyada oo aan loo eegin horumarka midabka on mid ka mid ah kooxaha T.Haddii kale, natiijadu waa sax, muunadana waa in lagu tijaabiyaa qalab kale.

NS1 Strip: Marka mug ku filan muunada shaybaadhka lagu shubo muunada ceelka cajalada, muunada waxa ay u guurtaa ficil capillary oo dhan cajalada tijaabada.Chikungunya NS1 Ag haday ku jirto muunada waxay xidhi doontaa isku xidhka Chikungunya Ab.Immunecomplex-ka ayaa markaa lagu qabtaa xuubka xuubka ka hortagga bakaylaha ee hore loo dahaadhay ee ka hortagga NS1, oo samaynaya koox T-midab leh oo burgundy ah, taasoo muujinaysa natiijada tijaabada togan ee Chikungunya Ag.Maqnaanshaha kooxda T waxay soo jeedinaysaa natiijo xun.Tijaabadu waxa ay ka kooban tahay kontorool gudaha ah (C band) kaas oo soo bandhigaya koox midab leh oo burgundy ah oo ah difaaca difaaca ee jiirka ka hortagga jiirka IgG/jiirka IgG-dahab iyada oo aan loo eegin joogitaanka kooxda T midabka leh.Haddii kale, natiijadu waa sax, muunadana waa in lagu tijaabiyaa qalab kale.