KOOBAN IYO SHARAXA IMTIXAANKA
Visceral leishmaniasis, ama Kala-azar, waa infekshan la faafiyay oo uu sababo dhowr nooc oo L. donovani ah.Cudurkan ayay hay’adda caafimaadka adduunka ee WHO ku qiyaastay in uu saameeyay ku dhawaad 12 milyan oo qof oo ku nool 88 waddan.Waxa u gudbiya bani-aadmiga qaniinyada ciidda-cad ee Phlebotomus, kuwaas oo caabuqa ka qaada quudinta xoolaha cudurka qaba.In kasta oo uu yahay cudur laga helo waddamada faqiirka ah, Koonfurta Yurub, waxa uu noqday cudurka ugu horreeya ee fursad-u-helidda bukaannada AIDS-ka.Aqoonsiga noolaha L. donovani ee dhiigga, dhuuxa lafta, beerka, qanjidhada qanjidhada ama beeryarada ayaa bixiya macne qeexan oo lagu ogaanayo.Ogaanshaha serological ee anti-L.donovani IgM waxaa la ogaaday inuu calaamad aad u fiican u yahay leishmaniasis ba'an ee Visceral.Tijaabooyinka lagu isticmaalo rugaha caafimaadka waxaa ka mid ah ELISA, fluorescent antibody ama baaritaanno agglutination oo toos ah 4-5.Dhawaan, ka faa'iidaysiga borotiinka gaarka ah ee L. donovani ee tijaabada ayaa si weyn u wanaajiyay dareenka iyo gaar ahaaneed.
Tijaabada degdega ah ee Leishmania IgG/IgM waa borotiinka dib-u-habaynta ku salaysan serological, kaas oo ogaanaya IgG iyo IgM unugyada difaacayaasha L. Donovani isku mar.Tijaabadu waxay ku siinaysaa natiijo la isku halayn karo 15 daqiiqo gudahood iyada oo aan wax qalab ah la helin.
MABADA
Tijaabada degdega ah ee Leishmania IgG/IgM waa immunoassay socodka chromatographic xaga danbe.Cajalka imtixaanku waxa uu ka kooban yahay: 1) suuf conjugate ah midabka burgundy oo ay ku jiraan recombinant L. donovani antigen oo isku xidha dahab kolloid ah (Leishmania conjugates) iyo bakaylaha IgG-dahab conjugates, 2) xabka nitrocellulose ah oo ka kooban laba qaybood oo tijaabo ah (T1 iyo T2 bands) iyo band control (C band).Kooxda T1 waxaa horay loogu dahaadhay monoclonal anti- bini'aadamka IgM si loo ogaado anti-L.donovani IgM, T2 band waxaa lagu dahaaray reagents si loo ogaado anti-L.donovani IgG, iyo kooxda C waxa lagu dahaadhay riyaha ka hortagga bakaylaha IgG.
Marka muunad ku filan muunada shaybaadhka lagu shubo muunada ceelka cajalada, muunada waxa ay u guurtaa ficilka capillary ee guud ahaan cajalada.L. donovani IgM haddii ay ku jirto muunada waxa ay xidhi doontaa isku xidhayaasha Leishmania.Immunecomplex-ka ayaa markaa lagu qabtaa xuubka xuubka kahortagga kahortaga bini'aadamka ee IgM antibody, samaynta koox T1 midab leh oo burgundy ah, taas oo muujinaysa natiijada baaritaanka togan ee L. donovani IgM.L. donovani IgG haddii ay ku jirto muunada waxa ay xidhi doontaa isku xidhayaasha Leishmania.Immunocomplex-ka ayaa markaa qabta reagents-ka hore loo dahaadhay ee xuubka, samaynta koox T2 midab leh, taas oo muujinaysa natiijada baaritaanka togan ee L. donovani IgG.
Maqnaanshaha koox kasta oo T ah (T1 iyo T2) waxay soo jeedinaysaa natiijo xun.Tijaabadu waxa ay ka kooban tahay kontorool gudaha ah (C band) kaas oo soo bandhigaya koox midab leh oo burgundy ah oo ah difaaca difaaca ee riyaha ka hortagga bakaylaha IgG/bakayle IgG-dahab iyada oo aan loo eegin horumarka midabka mid ka mid ah kooxaha T.Haddii kale, natiijadu waa sax, muunadana waa in lagu tijaabiyaa qalab kale.
